【摘 要】
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Insulators are non-coding gene regulatory elements in genome, which function as chromatin partitioning boundaries, and block interference across different c
【机 构】
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BioinformaticsDivision/CenterforSynthetic&SystemsBiology,TsinghuaNationalLaboratoryforInformationSci
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Insulators are non-coding gene regulatory elements in genome, which function as chromatin partitioning boundaries, and block interference across different chromatin domains.To facilitate modular design of synthetic gene circuit that is usually composed of multiple transcription units, unexpected regulatory interactions between different units should be avoided.Here, we functionally characterized the out-of-boundary regulatory interactions between different combinations of 6 promoters and 6 insulators.We showed that the interactions displayed a threshold-like effect, and insulators influenced the threshold position.Additionally, we tested the function of insulators in both cascade and toggle-switch circuits.The results showed that the out-of-boundary regulatory interactions were relatively weak compared to intended regulatory interactions in the synthetic systems, which might not cause strong deviation of working states in systems with open loops, saturated states and weak nonlinearity.
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