Background/purpose: UVR-induced immunosuppression leading to the development of skin cancers received more and more attention in recent years.This article attempts to investigate the immunoprotection of two different sunscreens on mice models,as well as whether skin Langerhans cells are involved in this process.Methods: Hydraphase XL sunscreen and Anthelios sunscreen were applied on back skin of BALB/c mice when treated with sub-erythema dose of solar-simulated radiation(SSR).Delayed type hypersensitivity was also induced by intraperitoneal injection with formalin-fixed Candida albicans.Then,swelling of the foot pads were measured,and immunosuppression rate was also evaluated.Expression of CD1a,CD80 and CD86 were semi-quantitatively detected by immunohistochemistry and Western-blot.Results: delayed type hypersensitivity mice model were successfully established.Immunosuppression rate of SSR group(73.0±11.3%)was higher than Hydraphase XL sunscreen(54.1±6.4%)and Anthelios sunscreen(29.7±7.5%)groups(P<0.01).Expression of CD1a and CD86 was decreased in SSR group.Whereas the expression of CD1a,CD86 and CD80 in sunscreen-treated group showed no significant difference comparing to SSR group.Conclusions: Sub-erythema of ultraviolet exposure can reduce Candida albicans antigen induced-delayed type hypersensitivity.Application of Hydraphase XL and Anthelios sunscreen can protect skin from immunosuppression.Epidermal Langerhans cells are not required for ultraviolet-induced immunosuppression.