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Little is known about endophytes associated with the grapevine plant inhabiting saline-alkali desertification Gobi.The diversity of endophytes was investigated from the root of Vitis vinifera collected from the Zixuan vineyard,located in Jiayuguan,Gansu,China.A total of 27 endophytes isolates(19 endophytic bacteria and 8 endophytic fungi)were isolated to further analyses.The endophytic bacteria and fungi were characterized with amplified ribosomal DNA restriction analysis and rDNA(ITS1-5.8S-ITS2)sequencing.Based on the identification methods,the isolates of bacteria and fungi were grouped into 9 genera and 5 genera,respectively.The main genera of bacteria are Amycolatopsis,Microbacterium,Streptomyces,Agrobacterium,Rhizobium,Pseudomonas,Enterobacter,Serratia,Staphylococcus and Bacillus,respectively.The main genera of fungi are Penicillium,Aspergillus,Achaetomium,Fusarium and Mucor,respectively.The results will lay the foundation for the research and development of functional strains in grapevine.Possibly,some endophytic isolates could be selected for the development of biological control agents for the growth of grapevine or grape fungal disease in vineyards.