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Triterpene glycosides from the sea cucumber Pearsonothuria graeffei showed excellent antitumor activities for several cancer cell lines,the IC50 values of crude glycosides were range from 8.17 to 10.78μg/ml.It should be noticed that the triterpene glycosides in this kind of sea cucumber is very abundant,the yield of crude glycosides was more than 100mg / g dry weight of Pearsonothuria graeffei.Eight triterpene glycosides were isolated and structure established from Pearsonothuria graeffei for the first time.