Characterization of Glycerol Phosphate Oxidase from Streptococcus pneumoniae and Its Application for

来源 :2015中国酶工程与糖生物工程学术研讨会 | 被引量 : 0次 | 上传用户:junr
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Glycerol phosphate oxidase from Streptococcus pneumoniae (GPOs.pne) was purified and characterized.Using the one-pot four-enzyme system we developed before (Scheme 1), a series of ketoses including four rare sugars were synthesized by replacing the commercial GPOS.the from Streptococcus thermophilus with GPOs.pne.The scale of the preparative synthesis is about 100 milligram in this study and the reaction system could be easily magnified if necessary.Considering the useful application of GPO in generating the expensive substrate DHAP, we believe this method could contribute to the preparation of more other useful carbohydrates and derivatives.
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