Background The aim of the study is to investigate the lung CD31CD45Sca-1+ cell quantity and Shh signaling pathway expression level in emphysema mice, and the effects of acute adenovirus infection on them.Methods BALB/c mice were interfereded with CSE intraperitoneal injection or adenovirus intratracheal instillation at certain timepoints within 28 days.We measured lung function, histomorphology of lung, lung CD31-CD45-Sca-1+ cell quantity and main components of Shh signaling pathway expression level in the lung.