Monolithic HPLC columns (Chromolith-columns) possess about 20% higher porosity than particulate ones.As a result they can be operated at much higher flow rates.A mixture of pharmaceutical drugs can be
LC/MS technique has become an important platform and also been widely used in the area of metabonomics.The "Ultra Performance" liquid chromatography (UPLC) employs alkyl-bonded porous particles with a
Recently tight junction (TJ) are considered as raft-like membrane compartments and represent a structural and functional distinct membrane microdomains, which are essential in spatial organization of
Amino acids play an important role in the metabolism of the human organism.Abnormal levels of amino acids suggest that certain metabolism pathway has been disrupted.For instance, phenyl ketonuria will
Plasma phospholipids metabolic profile of chronic glomerulonephritis was investigated.Nineteen phospholipid species were evaluated as potential biomarkers for chronic glomerulonephritis.High performan
As we known, RP-HPLC with ODS column is the most popular and efficient chromatographic mode because of the universal selectivity for most of the organic compounds, widely stationary phase supports, si
Now the researches on alkalophilic microorganisms mainly focus on the culture and enzyme extraction and there is few investigation of metabolic pathway.In this paper, Lp3-3 was selected as the experim
ODS columns typically possess a high level of hydrophobicity.This makes eluting a blood serum protein via an aqueous mobile phase very challenging.Some form of hydrophilic functional group is needed t
The core of metabonomic is to study the response of organism under extraneous or endogenic stimuli, which takes place in metabolism level.The objects of metabonomic study are the small molecules (meta
A selective, rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination and pharmacokinetic study of azithromycin in h