【摘 要】
：
Liver regeneration driven by hepatocyte proliferation is necessary for tissue repair and survival after acute liver injury,liver transplantation,and chronic hepatic disease.After a partial hepatectomy
【机 构】
：
第二军医大学医学遗传学教研室,上海,200433
【出 处】
：
中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议
论文部分内容阅读
Liver regeneration driven by hepatocyte proliferation is necessary for tissue repair and survival after acute liver injury,liver transplantation,and chronic hepatic disease.After a partial hepatectomy(PHx)in mice,a rapid induction of proliferative factors activates quiescent hepatocytes and primes their subsequent proliferation.During this process,many transcription factors are activated and up-regulate a large number of proliferation-related target genes to repair the liver.c-Myc is a central transcription factor and is essential for total liver regeneration.The up-regulation of c-myc mRNA occurs 30 minutes after PHx and sharply increases after several hours.However,the mechanism of the fast up-regulation of c-myc in early liver regeneration remains elusive.In this study,we reported for the first time that a c-myc-related positive feedback loop contributed to this phenomenon.A long noncoding RNA(lncRNA),H19,stabilized the c-myc mRNA by forming a H19-hnRNP U-myc complex and was transcriptionally up-regulated by c-myc in liver cells.Although many microRNAs,which are a type of noncoding RNA,have been reported to control liver regeneration,H19 is the first lncRNA that is involved in liver regeneration.We also found that H19 promotes an epithelial-to-mesenchymal transition(EMT)in liver cells during regeneration.Conclusion:lncRNA-H19 promotes hepatocyte proliferation and EMT by stabilizing c-myc mRNA in early liver regeneration.Moreover,the existence of a c-myc-related positive feedback loop augmented the effect of c-myc for a short time period during early liver regeneration.
其他文献
先天性心脏病(Congenital Heart Disease,CHD)是一种常见的先天畸形,由遗传因素与环境因素共同作用引起,其中遗传因素发挥重要作用,遗传率为55％～65％.心脏的发育过程受多种转录因子调控,其中T-box家族的TBX20基因参与调控心脏的增殖,分化以及心房的形成等,在心脏的发育过程中具有十分重要的作用.目的 研究靶向干扰Tbx20基因表达对心肌细胞凋亡和增殖的影响.方法 人工
In this report,we identified a homozygous missense mutation in a novel gene,TMEM132E (transmembrane protein 132E),in a chinese consanguineous family with nonsyndromic hearing loss using whole exome se
BACKGROUND Hypertensive disorders complicating pregnancy(HDCP)is one of the most common complication in obstetric.The real pathogenesis about HDCP is still unknown.Research had shown that the genesis
目的 探讨骨髓间充质干细胞(mesenchymal stem cells,MSCs)在体外模拟的肿瘤微环境中是否受到其中过度表达的致瘤性酪氨酸激酶信号分子的影响而发生恶性转化,为安全有效地利用MSCs提供一定的前期理论基础和实验依据.方法 本实验分筛选实验和验证实验两部分,筛选实验部分设为实验组,对照组和空白对照组三组.实验组为C6胶质瘤与MSCs间接共培养模拟肿瘤微环境下MSCs的生长；对照组为
目的 研究囊性纤维化转倒调节因子(cystic fibrosis transmembrane conductance regulator,CFTR)第8内含子中的5T等位基因多态性与中国汉族男性先天性输精管缺如(congenital bilateral absence of vas deferens,CBAVD)的相关性.方法 选择33例男性先天性双侧输精管缺如患者和99例对照普通无精症患者.提取
目的 食管鳞状细胞癌是我国十大恶性肿瘤之一,尤以男性高发.近年来,虽然临床对食管癌的诊治水平取得了显著进展,但是中晚期患者手术总体5年生存率仍然徘徊在10％左右.运用分子生物学技术检测基因及蛋白的改变,寻找灵敏度高、特异性强的分子标志物,被认为是改善食管癌诊断和治疗最有前景的手段之一.本研究旨在检测TIMP1蛋白在食管鳞癌中的表达情况,并探讨其在临床诊断与预后判断的应用价值.方法 应用组织微阵列联
High myopia,highly prevalent in the Chinese population,is a leading cause of visual impairment worldwide.Genetic factors play a critical role in the development of this visual disorder.Genome-wide ass
目的 阴道正常菌群对于维持阴道微生态环境的平衡起着十分重要的作用.以往采用的培养方法由于其本身技术的局限,无法获得相对准确的细菌谱的分布.本研究采用微生物组学方法,对中国健康育龄期汉族妇女阴道菌群的结构及其系统进化特征进行了初步研究,为进一步筛选和开发适合我国妇女生殖道健康的益生菌提供依据.方法 直接从阴道分泌物拭子中抽提细菌基因组DNA,采用细菌通用引物27f和1492r扩增16S rRNA基因
Background Massively parallel sequencing of DNA molecules in the plasma of pregnant women has been shown to allow noninvasive prenatal detection of fetal trisomy 21,18and13,could be a potential useful
Epithelial-Mesenchymal Transition(EMT)plays a pivotal role in tumor invasion and dissemination.Although it is well documented that EMT occurred predominantly at the tumor edge where it is induced by c