Objective: For targeted therapy of tumor angiogenesis is a hot research at home and abroad and anti-tumor drugs is an important development direction in the treatment of tumor angiogenesis, so to suggest or predict early treatment is important for guiding clinical intervention.Radionuclide labeled arginine-glycine-aspartic acid (RGD) peptide tracer binding to the integrin αvβ3 expressed on the surface of angiogenesis endothelial cells and tumor cell can demonstrate the tumor and prompt degree of expression of minor angiogenesis.In this study, we use 99mTc-3PRGD2 imaging to evaluate the effect of anti-angiogenesis drugs of recombinant human endostatin treatment in nude mice bearing human pancreatic cancer.Methods: 20 athymic nude mice at 6-8 weeks of age, weighing 20-25g, were implanted the suspension of pancreatic cancer PANC-1 organization into the right upper flanks.Animals with tumors about 200rmm3 were used in the experiment.Treatment of the tumor-bearing mice was divided into two groups.Endostar group: injection of Endostar (10mg/kg/200ml) was given in the peritumoral subcutaneous every other day.Control group: injection of 200ml saline was given in the peritumoral subcutaneous every other day in the peritumoral subcutaneous.SPECT Imaging was performed before treatment and in the 7,14,21,28 day after treatment, each animal was administered with 0.8 mCi of 99mTc-3PEG4-dimer.Static images were acquired atl.5h p.i.and stored digitally in a 128 × 128 matrix.Then we outlined the region of interest (ROI), calculate the tumor / health side of the corresponding parts of radioactivity (T / NT) ratio, and measure the tumor volume.In the 28st days, when imaging is completed, mice were killed, and the tumor weight inhibitory rate was calculated.Results: 99mTc-3PRGD2 can clearly show the tumor.Before treatment and 28 days later ,T / NT ratio, endostar group was 1.48 ± 0.15 and 1.81 ± 0.33, control group was 1.53 ± 0.36 and 2.52 ±0.59.After 28 days, the treatment group and control group s T / NT were significantly different (t =3.144, p <0.05).Before treatment and 28 days after treatment tumor volume, endostar group was 194.8 ± 9.4 and 1255.7 ± 265.8 mm3, control group was 150.4 ± 57.3 and 1706.5 ± 416.1 mm3 ,endostar can inhibit tumor growth (t =5.05, P <0.001).28 days after treatment, ratio of tumor inhibition was 22.1%Conclusions: Under experimental conditions, the growth of pancreatic tumor in nude mice significantly was inhibited compared with the control group, the slow growth of tumor, uptake of 99mTc-3PRGD2 less, T / NT ratio is low.99mTc-3PRGD2 imaging can be used to guide the pancreatic tumor angiogenesis drug treatment.