LTC4 synthesis enzymes including leukotriene C4 synthase (LTC4S), microsomal glutathione S-transferase (mGST) 2 and mGST3 can all conjugate LTA4 and reduced glutathione (GSH) to form LTC4, which involved hepatic ischemia-reperfusion (I/R) injury.To investigate the effects of NO on the gene expression of LTC4 synthesis enzymes during hepatic I/R.Adult male SD rats were divided into 3 groups: sham group (Control),I/R group and V-PYRRO/NO + I/R groups.Liver subjected to 1 h of partial hepatic ischemia followed by 5 h of reperfusion, saline or V-PYRRO/NO (1.06 μmol/kg/h) administered intravenously through all the experimental periods.The mRNA levels of LTC4 synthesis enzymes in rat liver tissue were examined by real time RT-PCR.We observed that hepatic mRNA expressions of LTC4S and mGST3 were lower whereas mGST2 mRNA was higher in V-PYRRO/NO +I/R group compared to I/R group.Compared with control, only mGST3 mRNA was significantly declined in V-PYRRO/NO +I/R groups.These results suggest that NO donor V-PYRRO/NO can down-regulate the mRNA expressions of LTC4S and mGST3 but up-regulate mGST3 mRNA during hepatic I/R injury.