【摘 要】
：
Membrane proteins present unparalleled challenges for structural biology initiatives,nascent functional genomics efforts,and drug discovery experiments.To overcome some of the current hurdles in obtai
【机 构】
：
Argonne National Laboratory USA
【出 处】
：
2008中国深圳蛋白质和多肽科学大会
论文部分内容阅读
Membrane proteins present unparalleled challenges for structural biology initiatives,nascent functional genomics efforts,and drug discovery experiments.To overcome some of the current hurdles in obtaining sufficient quantifies of membrane proteins for these studies,we are in the process of employing and optimizing a novel host/vector system based upon the Rhodobacter species of photosynthetic bacteria.This system exploits the unique physiology of this prokaryotic host whereby strongly-induced heterologous expression of the hydrophobic target proteins can be coordinated with induction of newly developing membranes,thereby favoring membrane insertion of natively folded polypeptides rather than inclusion body formation.
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