【摘 要】
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The genome organization of the two cultivated Canavalia species, C.gladiata and C.ensiformis, were characterized using sequential combined PI and DAPI (CPD)
【机 构】
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KeyLaboratoryofResearchandUtilizationofEthnomedicinalPlantResourcesofHunanProvince,HuaihuaUniversity
论文部分内容阅读
The genome organization of the two cultivated Canavalia species, C.gladiata and C.ensiformis, were characterized using sequential combined PI and DAPI (CPD) staining, dual-color fluorescence in situ hybridization (FISH) with 5S and 45S rDNA probes, and self-genomic in situ hybridization (self-GISH).For further assessing the genetic relationship between the two species, comparative genomic in situ hybridisation (cGISH) onto mitotic chromosomes was also performed.Detailed karyotypes of the two species were established for the first time using chromosome measurements, fluorochrome bands, as well as rDNA-FISH and self-GISH signals.Both species exhibited the same symmetrical karyotype formulated as n=11=11m (2SAT), but the karyotype length of C.gladiata was much larger than that of C.ensiformis.
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