Stressors can influence immune function by several potential mechanisms.To investigate the effect of cold stress on gut immune function in broilers,Sixty 15-d-old male chickens were randomly divided into 12 groups,and were kept under the temperature of (12±1) ℃ for acute and chronic cold stress.There were 1 control group and 5 treated groups for acute cold stress and 3 control groups and 3 treated groups for chronic cold stress.