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Ovarian cancer is one of the most serious malignant tumor in the female reproductive system.The prognosis of ovarian cancer is poor, therefore, it is important to search the new strategy of the clinical treatment in ovarian cancer.Gambogic acid (GA) is a kind of multiple targets of anti-tumor natural medicine, which play anti-tumor effect through various mechanisms.In this study, we researched the efficiency of GA in the treatment of ovarian cancer in vivo and in vitro.Our results found that GA inhibited the growth of ovarian cancer cells in a dose and time dependent manner.