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Objective.To overexpress CDT1 gene and investigate its effect on apoptosis andcell cycle in human liver cells that were genomic instability induced by radiation (GIR) Methods Lentivirus-mediated CDT1 gene overexpression system was constructedand verified its validity and infectious efficiency.The apoptosis was detected byAnnexin V-APC flow cytometry (FCM) and the cell cycle was determined by FCM withsingle PI staining.And the expression changes of p53,ATM, ATR, Bcl-2, Caspase3, Cyclin Egene were assayed by real time fluorescence quantitative PCR technology.