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论文目的：The aim of this study was to optimize the culture system of keratinocytes obtained from oral lichen planus (OLP) patients and verify whether this model could simulate the local inflammatory environment of OLP.论文方法：Keratinocytes were isolated from 48 OLP patients and cultured in vitro.The ultrastructure of OLP keratinocytes was observed via electron microscopy.The expression of pancytokeratin and vimentin was determined by immunohistochemistry, and the proliferation of OLP keratinocytes was measured by CCK-8 assay.Immunofluorescence staining was used to detect TLR4 and NF-K B p65 expression, and the levels.