【摘 要】
：
DHEA对骨代谢有重要的调节作用,表现为促进成骨细胞增殖、抑制成骨细胞凋亡、抑制破骨细胞的成熟等.然而,DHEA如何调节成骨细胞的分化仍不清楚.本研究中首先检测DHEA在体内对骨质疏松症模型小鼠骨组织形态的影响,发现DHEA增加骨密度,骨小梁数目,减小骨小梁间隙,说明DHEA明显改善骨微结构.体外实验中,检测DHEA对成骨细胞分化的影响以及成骨细胞形成相关基因Runx2,Osterix, Coll
【出 处】
：
2015全国中西医结合月经病专题学术会议
论文部分内容阅读
DHEA对骨代谢有重要的调节作用,表现为促进成骨细胞增殖、抑制成骨细胞凋亡、抑制破骨细胞的成熟等.然而,DHEA如何调节成骨细胞的分化仍不清楚.本研究中首先检测DHEA在体内对骨质疏松症模型小鼠骨组织形态的影响,发现DHEA增加骨密度,骨小梁数目,减小骨小梁间隙,说明DHEA明显改善骨微结构.体外实验中,检测DHEA对成骨细胞分化的影响以及成骨细胞形成相关基因Runx2,Osterix, Collagen1,Osteocalcin mRNA表达的影响,结果发现DHEA增加成骨细胞的生成,增加Runx2、Osterix、Collagen1、Osteocalcin mRNA表达.免疫细胞与骨之间存在相互作用,因此我们检测体内免疫细胞在药物治疗后的变化.DHEA增加脾脏FoxP3+调节性T细胞数目,但CTLA-4+ and IL-10+细胞无变化,且在调节性T细胞存在的条件下,DHEA促进成骨细胞的生成.尽管在成骨诱导培养条件下,DHEA增加脂肪细胞的数目,但在成脂诱导培养条件下,DHEA对脂肪细胞的数目及PPARr mRNA表达与对照组无差异,提示成骨细胞参与脂肪细胞的生成.因此认为,DHEA通过升高成骨细胞形成相关基因及FoxP3+调节性T细胞数目促进成骨细胞分化来预防绝经后骨质疏松症.
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