Treatment of Icaritin prevents amyloid-β (Aβ) peptide-induced aberrant AMPK phosphorylation and neur

来源 :第六届中国药理学会补益药药理专业委员会学术研讨会 | 被引量 : 0次 | 上传用户:wei_357
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Objective: This work aims to investigate the protective effect of icaritin (ICT) on amyloid-β peptide (Aβ)-induced neurotoxicity, and then to explore that if this effect of ICT is through increasing AMPK phosphorylation level in rats brain.Method: 40 SD rats of 18 months old were divided into contol group, model group, lower dosage of ICT group (20mg/kg), medium dosage of ICT group (40mg/kg), and high dosage of ICT group (80mg/kg) respectively.Model and treatment groups were injected with Aβ25-35 in rat hippocampus after being gavaged with ICT for 3 days, and then were gavaged with ICT for another 2 weeks.Learning and memory function of every rat was observed by water maze experiment, and the quantity of phosphorylation AMPK (pAMPK) in rat hippocampus was detected by ELISA, while the Aβ1-40 and pAMPK level in rat hippocampus were tested by Western Blot.
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