【摘 要】
：
该研究以矮脚黄白菜核不育两用系的测交后代群体为实验材料,利用AFLP指纹图谱技术研究了可育系与不育系基因组DNA多态性差异,并对差异条带进行了克隆、测序.探讨了所得差异片
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该研究以矮脚黄白菜核不育两用系的测交后代群体为实验材料,利用AFLP指纹图谱技术研究了可育系与不育系基因组DNA多态性差异,并对差异条带进行了克隆、测序.探讨了所得差异片段与先前所得的育性相关基因之间的关系.结果如下:(1)该实验分别以可育系和不育系基因组DNA混合池为模板,选用64对E+3/M3-引物组合进行选扩筛选,分析得到了两条可育系所特有的差异条带MF-14和MF-24.(2)以回收得到特异片段MF-14为探针,通过Southern杂交检测到白菜不育系中也含有该片段的同源序列.并初步探讨不育系碱基序列突变情况.(3)序列测定表明特异片段MF-14长289bp;比较特异片段MF-14与先前所得育性相关片段S107及育性相关基因CYP86MF、BCMF1、BCMF2、BCMF3、BCMF4之间序列关系,结果显示它们之间的相似性均小于50﹪.分析结果表明MF-14是可育系中发现的一个新的特异片段.(4)以MF-14特异片段为基础,设计引物进行了SCAR标记转换研究.
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