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目的:本研究利用野生型3号染色体的不同基因片段缺失的7日龄黑腹果蝇(Drosophila melanogaster)探讨影响果蝇睡眠的基因片段。方法:1.以野生型3号染色体的不同基因片段缺失的品系(6867,7443,8047,8049,8061,8965,8967,24627,24968,25126,26524,30589,9701,9082,23674,7594,24386,6755,26533,26836,8068,997,26523,7562,8098,6964,6457,24971,27577,8073,8100,8099,8974,8074,8066,24392,7595,9700,26828,24415,7737,9482,8072,3650,6646,8097,7413,30592,8069,26846,25019,25116,27369,24990,27917,8957,9697,9481,25724,8104,27888,9501,7983,8964,8970,3486,27580,6962,7002,9500,24909,24965,7681,24413,24139,6367,2597,8103,24980,25077,9077,25006,25011,25007,25005,25008,9090和8058)的7日龄黑腹果蝇(Drosophila melanogaster)为实验对象,利用果蝇活动监测系统(Drosophila Activity Monitoring System, DAMS),通过与野生型Canton S (CS)品系的同日龄黑腹果蝇相比较,以24h内睡眠总时间(Total Sleep Time, TST)为观察指标,确定影响果蝇睡眠总时间的基因片段。2.选择上述实验中影响睡眠总时间的野生型3号染色体的不同基因片段缺失的7日龄黑腹果蝇(Drosophila melanogaster),利用上述方法进行重复性实验,以确保实验结果的准确、可信。结果:1.野生型3号染色体的基因片段缺失的7443,8047,8049,8061,8965,8967,24627,24968,25126,26524,30589,9701,9082,7594,24386,6755,26533,26836,8068,997,26523,7562,8098,6964,6457,24971,27577,8073,8100,8099,8974,8074,8066,7595,9700,26828,24415,9482,8072,6646,8097,8069,26846,25019,25116,27369,24990,9697,9481,25724,8104,27888,9501,8964,8970,3486,7002,9500,24909,7681,24413,24139,6367,2597,8103,24980,25077,9077,25006,25011,25007,25005,25008,9090和8058品系雌、雄黑腹果蝇与野生型CS品系雌、雄黑腹果蝇相比,24h内睡眠总时间无显著性差异(P>0.05)。2.野生型3号染色体的基因片段缺失的6867,23674,24392,7737,3650,7413,30592,8957,27580,6962和24965品系雄性黑腹果蝇与野生型CS品系雄性黑腹果蝇相比,24h内睡眠总时间明显增加,具有极显著性差异(P<0.001)。3.野生型3号染色体的基因片段缺失的6867,23674,24392,7737,3650,7413,30592,8957,27580,6962,24965,27917和7983品系雌性黑腹果蝇与野生型CS品系雌性黑腹果蝇相比,24h内睡眠总时间明显增加,具有极显著性差异(P<0.001)。4.重复性实验研究结果表明:野生型3号染色体的基因片段缺失的6867,23674,24392,7737,3650,7413,30592,8957,27580,6962和24965品系雄性黑腹果蝇与野生型CS品系雄性黑腹果蝇相比,24h内睡眠总时间明显增加,具有极显著性差异(P<0.001);野生型3号染色体的基因片段缺失的6867,23674,24392,7737,3650,7413,30592,8957,27580,6962,24965,27917和7983品系雌性黑腹果蝇与野生型CS品系雌性黑腹果蝇相比,24h内睡眠总时间明显增加,具有极显著性差异(P<0.001)。结论:1. Df(3L)BSC27、 Df(3L)BSC23、 Df(3L)BSC368、 Df(3R)Exe16270、 Df(3L)M1、Df(3R)BSC43、 Df(3R)BSC887、 Df(3R)ED5514、 Df(3R)BSC819、 Df(3R)ED2和Df(3R)BSC461基因片段对雌、雄黑腹果蝇都有影响,均能延长果蝇的睡眠总时间。2. Df(3L)BSC839和Df(3R)Exe17328基因片段对雌性黑腹果蝇有影响,均能延长果蝇的睡眠总时间,但对雄性黑腹果蝇没有影响。