Raman spectroscopy is a common spectral analysis method for detecting the chemical bonds, symmetry or other chemical composition and structural information of specimen.As it can provide the spectroscopic chemical information of material, the conventional Raman spectroscopy has been wildly utilized in fields of physics,chemistry, material science, biology, medicine, and environmental science.In nano science and nano technology, acquiring of the chemical composition of surfaces and interfaces with high spatial resolution and high sensitivity is very important.The ability to do so would facilitate advances in the fields of surface chemistry, biology and materials sciences.To achieve this goal, the ability to perform in situ and nondestructive chemical analysis on samples with nanometer spatial resolution is demanded.Tip-enhanced Raman spectroscopy (TERS) overcomes the difficulties and obtains optical resolution beyond the diffraction limit and remarkable signal enhancement in spectral and Raman detection in the nanometer scale.In this thesis, we plan to fabricate AFM tip by metal films for tip enhanced Raman spectroscopy (TERS) measurement.To acquire the metalized AFM tip for TERS, the nano metal films should be ingenious coated onto the apex of a top-view Si-AFM tip with suitable thickness and roughness.With the metalized AFM tips, self-assembled monolayer of PhS and the thin film BCB on gold film will be measured by TERS.After that, we also plan to utilized TERS to measure the secondary structure of the rod/ring-like aggregates of Aβ(1-40) and the strains of near edge and angle of single layer graphene.The srains are caculated by recording the shift of G position (resulted from stress) by TERS.The main contents of this thesis contain the following three parts:(1) Preparation of metalized AFM tip for TERS measurement.In this part, we report a fabrication protocol for AFM-TERS tips that incorporate a copper (Cu) primer film between a gold (Au) layer and a Si AFM tips.They were fabricated by coating the Si tip with a 2 nm Cu layer prior to adding a 20 nm Au layer.For top illumination TERS experiments, these tips exhibited superior TERS performance relative to that observed for tips coated with Au only.Samples included graphene,thiophenol and brilliant cresyl blue.The results may derive from the surface roughness of the tip apex and a Cu/Au synergism of local surface plasmon resonances.(2) TERS measurement of ring-like and rod-like aggregation of Aβ(1-40).In this part we introduce molecular chirality at liquid-solid interfaces and report an interesting ring-like aggregation of Aβ(1-40) on N-isobutyryl-L-cysteine (L-NIBC)modified gold substrate at low Aβ(1-40) concentration, while D-NIBC modification only results in rod-like aggregation.Utilizing atomic force microscope controlled tip-enhanced Raman scattering, we directly observe the secondary structure information for Aβ(1-40) assembly in situ at nanoscale.We find that D-or L-NIBC on surface can guide parallel or nonparallel alignment of β-hairpin through a two-step process based on electrostatic interaction enhanced adsorption and subsequent stereoselective recognition.We propose the possible electrostatic interaction sites (R5 and K16) and chiral recognition site (H14) of Aβ(1-40), which may provide insight into the understanding of this effect.(3) TERS measurement of the strain of single layer graphene.In this part, we utilize TERS to study the properties of the nano structure of single layer graphene.The results of this study illustrate that the exceptional spatial resolution of TERS allows spectroscopic measurements of individual nanostructures.By analyzing TERS spectra, the change of local strain on the edge/angle and flat area were detected.Using G band positions in the TERS spectra, the strain difference between the edge/angle and flat area was great different for uniaxial and biaxial strain, respectively.With this study, we demonstrate that TERS is a powerful technique for the characterization of strain of individual local nanostructures of graphene.