论文部分内容阅读
对体外培养新生SD大鼠心房壁的神经细胞，按SternbergerPAP法进行了降钙素基因相关肽（CGRP）和神经肽Y（NPY）免疫细胞化学研究。结果显示：CGRP-IR阳性细胞数量较少，实验的5份标本只有2份见到阳性细胞，而NPY-IR阳性细胞在所有实验标本中均可见到。本研究在培养条件下证实心内神经节中有CGRP-IR阳性细胞存在，支持心脏有器官内感觉神经细胞的设想。同时对心内神经细胞的化学性质进行了讨论。
The neuronal cells of atrial wall of neonatal SD rats were cultured in vitro, and CGRP and NPY immunocytochemistry were studied by SternbergerPAP method. The results showed that the number of CGRP-IR positive cells was small, only 2 of the 5 samples showed positive cells, while the NPY-IR positive cells were seen in all the experimental samples. This study confirmed that there are CGRP-IR positive cells in the cardiac ganglia under the culture conditions, which supports the hypothesis that the heart has sensory neurons in the organs. The chemistry of intracardiac nerve cells was also discussed.