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目的:研究川楝子对大鼠肝组织超微结构和原代培养肝细胞的影响。方法:大鼠口服川楝子提取液(生药)90 g.kg-1,2 h后取肝组织,电镜下观察肝组织超微结构变化。分离培养原代大鼠肝细胞,加入川楝子含药血清共同孵育,分别于1,2,8,24 h时检测培养上清液中LDH,ALT,AST水平。结果:大鼠口服川楝子提取液后2 h,肝细胞体积增大,线粒体肿胀变性,内质网扩张,胞浆内脂滴比正常组略有增多。5%或10%川楝子含药血清均可导致肝细胞上清液中LDH,ALT,AST水平不同程度的升高(P<0.05或P<0.01),在24 h最为显著。结论:川楝子口服能被机体吸收,其原型药物或代谢产物对肝细胞产生直接的损伤作用。
Objective: To study the effect of Toosendan on ultrastructure of liver tissue and primary cultured hepatocytes in rats. Methods: The rats were orally administered with extract of Toosendan sinensis (crude drug) at 90 g.kg-1, 2 h, and the liver tissue was taken out. The ultrastructure of liver tissue was observed under electron microscope. The primary rat hepatocytes were isolated and cultured, and the serums of Toosendan seeds were added to incubate. The levels of LDH, ALT and AST in culture supernatants were detected at 1, 2, 8 and 24 h. Results: After oral administration of Toona sinensis extract for 2 h, the volume of hepatocytes increased, the mitochondria became swollen and denatured, the endoplasmic reticulum was dilated and lipid droplets in the cytoplasm slightly increased compared with the normal group. Serum of 5% or 10% Toona sinensis could cause LDH, ALT and AST levels in liver cell supernatant to increase to different extents (P <0.05 or P <0.01), and was most significant at 24 h. Conclusion: Toosendan can be absorbed by the body orally, and its prototype drugs or metabolites have a direct damage to the liver cells.