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目的探讨应用组织芯片技术对FHIT基因在90例生存期肝癌手术标本中的表达差异.方法收集2010年1月至2013年9月90例临床资料和随访记录完整的原发性肝癌患者的手术标本,以相应的癌旁组织为对照,以免疫组化染色制作组织芯片,观测上述标本中FHIT基因表达差异.结果 FHIT基因在本实验组的人肝癌手术标本中的低表达是高概率事件(67/90;74.4%),同时癌旁组织的FHIT基因表达显著高于肝癌细胞(P<0.05)且组织芯片免疫组化染色定量分析FHIT基因在本实验组的肝癌组织和癌旁组织中的表达差异有统计学意义(P<0.05).结论 FHIT基因是一种抑癌基因,其在肝癌细胞中的低表达可提示肿瘤恶性程度增加,免疫组化检测FHIT基因有助于肝癌的恶性程度判断和术后生存期预后的合理评估.
Objective To investigate the differential expression of FHIT gene in 90 surgical specimens of hepatocellular carcinoma (HCC) treated with tissue microarray technique.Methods 90 surgical specimens from January 2010 to September 2013 were collected, and the surgical specimens of patients with primary liver cancer , And the corresponding paracancerous tissues were used as control to make tissue microarray by immunohistochemistry to observe the difference of FHIT gene expression in the above specimens.Results The low expression of FHIT gene in the specimens of human hepatocellular carcinoma in this experimental group was a high probability event / 90; 74.4%). Meanwhile, the expression of FHIT gene in paracancerous tissues was significantly higher than that in hepatocellular carcinoma cells (P <0.05), and the expression of FHIT gene in hepatocellular carcinoma tissues and paracancerous tissues was quantitatively analyzed by tissue microarray immunohistochemical staining (P <0.05) .Conclusion FHIT gene is a tumor suppressor gene, its low expression in liver cancer cells can be prompted to increase the degree of malignancy, immunohistochemical detection of FHIT gene contribute to the degree of malignancy of liver cancer And postoperative survival of a reasonable assessment of the prognosis.