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比较了上海市农业科学院作物育种栽培研究所培育出的花培15、秋丰、香粳834三个品种幼胚在光照和黑暗培养条件下愈伤组织的诱导频率，发现在同一培养条件下，不同品种幼胚愈伤组织诱导频率差异显著。在不同培养条件下，同一品种幼胚愈伤组织诱导频率差异不明显。但光照可显著提高幼胚的愈伤组织质量。将来自光照和黑暗条件下培养8～20d诱导的花培15幼胚愈伤组织置于添加不同激素的分化培养基上，发现光照条件下诱导的幼胚愈伤组织，更易分化，其分化频率显著高于黑暗条件下诱导的愈伤组织。添加6－BA2mg／L、NAA0.5mg／L、KT0.5mg／L的MS培养基最适合花培15幼胚愈伤组织的分化再生。经多次继代的花培15幼胚愈伤组织的分化能力显著低于初培养8～20d的愈伤组织。用基因枪法将含Act15’端调控的bar基因导入花培15、94－J－21、94－J－09、94－J－18、94－J－24等5个品种（系）预培养3d的幼胚。转化的幼胚在含PPT（Glufosinateammonium）4mg／L的MS培养基上诱导愈伤组织。抗性愈伤组织经2～3次继代后，转移至添加PPT4mg／L分化培养基M3上分化再生。在转化的5个品种中，仅获得花培15的抗性再生植株。
The frequency of callus induction of three cultivars of Huapei 15, Qiufeng and Xiangjing 834 cultivated in the Institute of Crop Breeding and Cultivation of Shanghai Academy of Agricultural Sciences was compared under light and dark culture conditions. The results showed that under the same culture conditions, Different varieties of immature embryos callus induction frequency significantly different. Under different culture conditions, there was no significant difference in callus induction frequency among the same variety of immature embryos. But light can significantly improve the callus quality of immature embryos. The callus from flower bud 15 induced by 8 ~ 20d culture under light and dark conditions was placed on differentiation medium with different hormones. The immature embryo callus induced under light irradiation was found to be more differentiated, and its differentiation frequency Significantly higher than those induced under dark conditions. MS medium supplemented with 6-BA2mg / L, NAA0.5mg / L and KT0.5mg / L was the most suitable for the differentiation and regeneration of the embryos cultured in vitro. After multiple subcultures, the differentiation ability of the immature embryos was significantly lower than that of the callus cultured for 8-20 days. The bar gene with Act15 ’end regulated by gene gun was introduced into 5 varieties (lines) of flower cultivars such as flower cultivar 15,94-J-21,94-J-09,94-J-18,94-J-24 Immature embryos. The transformed immature embryos induced callus on MS medium containing 4mg / L Glufosinatemonium (PPT). After 2 ~ 3 subcultures, the resistant calli were transferred to M3 supplemented with 4 mg / L PPT for differentiation and regeneration. Among the five cultivars that were transformed, only regenerated plants with flower culture 15 were obtained.