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目的 用异硫氰荧光素(FITC)-右旋糖苷和四甲基若丹明(TMR)-右旋糖苷标记小鼠2-细胞胚胎的两个卵裂球,观察其发育,以探讨囊胚Em-Ab极性的形成.方法 向受精卵注射FITC-右旋糖苷确定标记物对胚胎的伤害性,向2-细胞胚胎两个卵裂球分别注射FITC-右旋糖苷和TMR-右旋糖苷,将标记后的胚胎体外培养发育至囊胚,观测两个卵裂球后代在囊胚中的分布情况.结果 2-细胞胚胎两个卵裂球的后代在囊胚中分布并无规律.结论 2-细胞胚胎卵裂球随机分布在囊胚的胚胎部分和胚外部分.“,”Objective Kunming strain(KM) mice were used as animal models. Nontoxic dextran conjugated with tetramethylrhodamine(TMR)and fluorecein isothiocyante(FITC)was microinjected to two of the 2-cell blastomere as molecular probe to trace the development fate of the blastomere ,in order to figure out the mechanisms of the formation of Em-Ab axis. Methods FITC- dextran was injected to zygote in order to make sure if it is noxious. Two blastomeres of 2-cell embryo were injected FITC- dextran and TMR- dextran respectively. Results When labeled embryo develeped to blastocyst, distribution of progeny of 2-cell embryo blastomeres can be detected.Conclusion The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.