论文部分内容阅读
目的 :血灵是降血压中药制剂 ,本实验探讨血灵对心血管中枢的作用及其机理。方法 :将药物微量地注射入麻醉大鼠侧脑室 ,观察注药后 60min内血压和心率的变化。结果 :①侧脑室注射血灵 ( 1 0mg/2 0 μl)引起动脉血压明显升高 ,2 5min时达到峰值 ,增幅为 2 6.59% ;心率增加 ,增幅为 1 5.76%。侧脑室注射相同容积的NS ,血压和心率无明显影响。②血压升高与侧脑室注射血灵的剂量呈明显的剂量 -效应关系。③侧脑室注射血灵致血压和心率的增加效应可被侧脑室内预先注射 β受体阻断剂普萘洛尔 ( 2 0 μg/2 0 μl)或M受体阻断剂阿托品 ( 1 0 μg/2 0 μl)拮抗 ,但不能被α受体阻断剂酚妥拉明 ( 2 5μg/2 0 μl)拮抗。 结论 :血灵具有中枢性升高动脉血压和心率作用 ,该作用可能部分地由脑内的 β受体和M受体介导。进而推测血灵的降压效应是通过心血管系统的外周活动起作用的。
Objective: Blood pressure is a hypotensive traditional Chinese medicine preparation. This experiment explores the effect of blood pressure on cardiovascular center and its mechanism. Methods: The drug was injected into the lateral cerebral ventricle in anesthetized rats, and the changes of blood pressure and heart rate were observed within 60 minutes after injection. Results: (1) Intraventricular injection of blood spirit (10mg / 2 0 l) caused by arterial blood pressure was significantly increased, reached a peak at 25min, an increase of 2 6.59%; heart rate increased by an increase of 15.76%. Intracerebroventricular injection of the same volume of NS, blood pressure and heart rate had no significant effect. ② blood pressure and intraventricular injection of blood Ling dosage was significantly dose-response relationship. ③ Intracerebroventricular injection of blood-induced blood pressure and heart rate increases can be pre-injection of intracerebroventricular injection of β-blocker propranolol (20μg / 2 0 μl) or M receptor blocker atropine (10 μg / 20 μl), but not by the α-blocker phentolamine (25 μg / 20 μl). CONCLUSIONS: Blood has a central elevation of arterial blood pressure and heart rate, which may be mediated, in part, by beta receptors and M receptors in the brain. And then speculated that the antihypertensive effect of blood pressure through the cardiovascular system’s peripheral activities play a role.