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方法:将人的麻风结节(活组织标本15份)和冰冻干燥保存10年的鼠麻风菌(包括:夏威夷、警视厅、福冈、巴斯得、熊本,久留米、答姑拉斯等7个菌株)分别接种于出生4周的雌性 ddF 系小白鼠的下腹部和右侧足垫部。接种3个月后采取接种部位的皮肤组织制成石腊标本。脱腊后用62℃无水吡啶处理24、48小时和5、7天。除去吡啶后在室温条件下作抗酸染色(Ziehl-Hematoxylin 法)进行比较。结果:含有人麻风菌的组织标本经吡啶处理24小时后,15份标本中有13份抗酸染色阴性,经吡啶
Methods: The human leprosy nodules (15 specimens of living tissue) and leprosy (including: Hawaii, Metropolitan Police Department, Fukuoka, Bath, Kumamoto, Kurume, Tauranga et al.7 Strains) were inoculated into the lower abdomen and the right footpad of female ddF-type mice of 4 weeks old, respectively. Three months after inoculation, the skin tissue at the inoculation site was used to make paraffin wax specimens. Dewaxing with 62 ℃ anhydrous pyridine treatment 24,48 hours and 5,7 days. The pyridine was removed and compared with acid-resistant staining (Ziehl-Hematoxylin method) at room temperature. RESULTS: Twenty-three of the 15 samples were negative for acid-fast staining after 24 hours of treatment with tissue samples of the human leprosy. After pyridine