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近年来快速发展的微流控技术为单细胞基因诊断的基础研究提供了一个有力的平台,微流控芯片具有样品和试剂低消耗、快速实时、大量样本平行处理等优点,还可以使分析过程自动化、防止污染以及完成自动高效的重复实验。本文主要介绍一种用于高通量单细胞基因表达水平分析的微流控芯片技术平台。芯片集成了单细胞捕获、裂解、纯化、逆转录等生物样品处理单元,通过内置气动微阀实现细胞反应腔的独立寻址,从而实现单细胞处理与分析整个过程的自动化操控。实验结果显示了此平台可以实现基因表达水平稳定性的评估,具有应用于肿瘤早期诊断领域的潜力。
In recent years, the rapid development of microfluidic technology for single-cell gene diagnosis of basic research provides a powerful platform for microfluidic chips with low consumption of samples and reagents, fast real-time, a large number of sample parallel processing, etc., but also can make the analysis process Automate, prevent contamination and complete automated, repeatable experiments. This article describes a microfluidic chip technology platform for high-throughput single-cell gene expression profiling. The chip integrates the single-cell capture, lysis, purification, reverse transcription and other biological sample processing unit, through the built-in pneumatic micro-valve to achieve independent access to the cell reaction chamber in order to achieve single-cell processing and analysis of the entire process of automated control. The experimental results show that this platform can evaluate the stability of gene expression level and has the potential to be applied in the field of early diagnosis of tumors.