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通过摇瓶液体培养实验,确定江西虫草培养基为蔗糖2%,甘油1%,黄豆浸液0.5%,酵母膏0.5%,KH2PO40.1%,MgSO40.05%,蒸馏水1000mL,初始pH6～7。装液量200～250mL 500mL,加入10粒分散球,按培养液量的5%～7.5%接种6d左右菌龄的菌种,于26～28℃、旋转式摇床150～180r min培养8d,最大菌丝体生物量可达16.6g L。
The liquid culture experiments of shake flask showed that the culture medium of Cordyceps mongolicus was 2% sucrose, 1% glycerol, 0.5% soy bean extract, 0.5% yeast extract, 40.1% KH2PO4, 0.05% MgSO4, 1000mL distilled water and initial pH6-7. The amount of liquid was 200 ~ 250mL 500mL, 10 dispersed balls were added and inoculated with 5 ~ 7.5% of the culture solution for 6 days or so bacteria culture at 26 ~ 28 ℃, rotary shaker 150 ~ 180r min for 8 days, The maximum mycelium biomass up to 16.6g L.