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目的观察乌苯美司对肾癌细胞增殖及死亡的影响并探讨其可能机制。方法利用WST-8细胞增殖实验及细胞生长曲线检测乌苯美司对肾癌细胞增殖能力的影响;LDH释放实验检测乌苯美司对肾癌细胞死亡的影响;酶活性测定法及Western blotting研究乌苯美司诱导肾癌细胞APN活性及表达;Western blotting及免疫组化染色观察乌苯美司的作用靶点氨肽酶N(APN)/CD13在肾癌组织及正常组织中的表达。结果乌苯美司不能降低肾癌细胞中APN的表达但是可以抑制其活性;乌苯美司可抑制肾癌细胞增殖及活力,诱导细胞自噬性死亡。结论乌苯美司可抑制肾癌细胞增殖,并可通过诱导自噬引发肾癌细胞死亡。
Objective To observe the effect of ubenimex on proliferation and death of renal cell carcinoma and to explore its possible mechanism. Methods WST-8 cell proliferation assay and cell growth curve were used to detect the effect of ubenimex on the proliferation of renal cell carcinoma cells. LDH release assay was used to determine the effect of ubenimex on renal cell carcinoma cell death. The enzyme activity assay and Western blotting Ubenimex induced APN activity and expression in renal cell carcinoma cells. The expression of aminopeptidase N (APN) / CD13, a target of ubenimex, in renal cell carcinoma and normal tissues was observed by Western blotting and immunohistochemistry. Results Ubenimex could not reduce the expression of APN in renal cancer cells but could inhibit its activity. Ubenimex could inhibit the proliferation and viability of renal carcinoma cells and induce autophagic cell death. Conclusion Ubenimex can inhibit the proliferation of renal cell carcinoma and induce the death of renal cell carcinoma by inducing autophagy.