论文部分内容阅读
本文基于PC12细胞模型研究大豆蛋白水解物(Soybean protein isolate hydrolysates,SPIHs)对神经元氧化损伤的保护作用。以大豆蛋白为原料,经过酶解和膜分离得到四种分子量不同的水解物,我们首先检测了SPIHs的抗氧化能力;然后用H2O2刺激P12细胞,建立神经元氧化损伤模型,并以适当浓度的SPIHs处理细胞,通过检测各种生物学指标评价对细胞氧化损伤的保护作用。结果显示,低分子量的SPIHs表现出最强的抗氧化活性;能够提高损伤细胞的存活率和抗氧化酶活力,减少乳酸脱氢酶(LDH)的释放量和丙二醛(MDA)的生成,抑制细胞活性氧(ROS)的累积(p<0.05或p<0.01),且变化呈现一定的剂量依赖关系。研究认为,低分子量的SPIHs对神经元氧化损伤具有保护作用,可以作为功能性成分用于保护神经元氧化损伤相关的功能食品和保健品的开发。
In this paper, the protective effects of Soybean protein isolate hydrolysates (SPIHs) on oxidative damage of neurons were studied based on PC12 cell model. Using soybean protein as raw material, four hydrolyzates with different molecular weights were obtained by enzymolysis and membrane separation. We first detected the antioxidant capacity of SPIHs. Then, P12 cells were stimulated with H2O2 to establish a neuronal oxidative damage model. SPIHs cells, through the detection of various biological indicators to evaluate the protective effect of cell oxidative damage. The results showed that the low molecular weight SPIHs showed the strongest antioxidant activity, which could improve the survival rate of injured cells and antioxidase activity, reduce the release of lactate dehydrogenase (LDH) and the formation of malondialdehyde (MDA) Inhibited the accumulation of reactive oxygen species (ROS) (p <0.05 or p <0.01), and showed a dose-dependent manner. Studies suggest that low molecular weight SPIHs have a protective effect on neuronal oxidative damage and can be used as a functional component in the development of functional foods and health supplements related to the protection of neuronal oxidative damage.