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目的：探讨白细胞与内皮细胞粘附对内皮单层通透性的影响。方法：将兔肺微血管内皮细胞单层培养在通透性测定装置内室底部的微孔滤膜上，然后与兔多形核白细胞共育，通过ＣＤ１８ 单抗封闭试验，观察缺氧时兔多形核白细胞与内皮细胞粘附率、［１２５Ｉ］ － 白蛋白清除率、培养基中ＰＡＦ 与ＮＯ 的变化及其相关关系。结果：缺氧时［１２５Ｉ］ － 白蛋白清除率、培养液中ＰＡＦ 与ＮＯ 水平与白细胞－ 血管内皮细胞的粘附有显著相关性。结论：缺氧时内皮单层通透性部分依赖于白细胞－ 血管内皮细胞的粘附，并与粘附所致的ＰＡＦ、ＮＯ 释放有关。
Objective: To investigate the effect of leukocyte and endothelial cell adhesion on endothelial monolayer permeability. Methods: Monolayers of rabbit pulmonary microvascular endothelial cells were cultured on the microporous membrane at the bottom of the cell in the permeability measuring device, co-cultured with rabbit polymorphonuclear leukocytes, and then tested by CD18 monoclonal antibody blocking test. Adherent ratio of nucleated leucocytes to endothelial cells, [125I] -albumin clearance, changes of PAF and NO in culture medium and their correlation. Results: The levels of [125I] -albumin clearance, PAF and NO levels in culture medium were significantly correlated with leukocyte-endothelial cell adhesion in hypoxia. CONCLUSIONS: Hypoxia partially suppresses endothelial monolayer permeability by leukocyte-endothelial cell adhesion and is associated with adhesion-induced release of PAF and NO.