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本法是基于五氰亚硝酰铁酸二钠(diso—dium pentacyanonitrosylferrate与脂肪族伯胺和仲胺的Rimini反应,可用以测定丁胺卡那霉素、卡那霉素、新霉素和妥布拉霉素;但对该四个抗生素没有分辨能力。该四种抗生素的浓度与反应后在540nm时的吸收峰值符合Beer定律:其适当浓度分别为2.8×10~(-4)～1.7×10~(-3)mol/L、2.6×10~(-4)～2.0×10~(-3)mol/L、1.7×10~(-4)～1.9×10~(-3)mol/L和2.5×10~(-4)～1.6×10~(-3)mol/L。
This method is based on the Rimini reaction of diso-dium pentacyanonitrosylferrate with aliphatic primary and secondary amines and can be used to determine amikacin, kanamycin, neomycin, and However, the four antibiotics did not have resolving power.The concentrations of these four antibiotics and the absorbance peak at 540 nm after the reaction accorded with Beer’s law: the appropriate concentrations were 2.8 × 10 -4 -1.7 × The results showed that there was no significant difference between the experimental group and the control group (P <0.05) L and 2.5 × 10 ~ (-4) ~ 1.6 × 10 ~ (-3) mol / L.