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目的:建立风痛宁片中士的宁的RP-HPLC测定方法。方法:采用DiamonsilTM C18(钻石)色谱柱(250mm×4.6mm,5μm),以乙腈-0.01mol/L庚烷磺酸钠与0.02mol/L磷酸二氢钾等量混合溶液(10%磷酸调节pH值2.8)(79.5:20.5)为流动相;检测波长254nm。结果:平均加样回收率为100.2%,RSD=1.3%(n=5),线性范围为0.1～1.0μg/mL。结论:该方法重复性好,结果准确,可用于该制剂的质量控制。
Objective: To establish a RP-HPLC method for the determination of strychnine in Fengtongning tablets. Methods: DiamonsilTM C18 (diamond) column (250mm × 4.6mm, 5μm) was used to prepare a mixed solution of acetonitrile-0.01mol / L heptane sulfonate and 0.02mol / L monobasic potassium phosphate Value of 2.8) (79.5: 20.5) as the mobile phase; detection wavelength 254nm. Results: The average recovery was 100.2% with RSD = 1.3% (n = 5) and the linear range was 0.1-1.0 μg / mL. Conclusion: The method has good repeatability and accurate results and can be used for the quality control of the preparation.