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探讨丙烯腈对雄性小鼠生殖细胞的毒性作用。 [方法 ]雄性ICR小鼠以 0、2 5、5、10mg/kg体重剂量连续腹腔注射染毒 5d ,2周后处死动物 ,观察睾丸中早期精细胞微核 ;ICR雄性小鼠以 0、6、12、2 4mg/kg体重连续灌胃染毒 5d后于每周末处死部分动物 ,连续 5周 ,分析附睾精子畸形率。 [结果 ]早期精细胞微核实验结果表明 ,染毒剂量≥ 5 0mg/kg ,早期精细胞微核明显增加 ,微核率与染毒剂量之间存在剂量反应关系 (y =0 2 70x- 0 816 ,r=0 988)。精子畸形实验结果表明 ,第 2周起 2 4mg/kg组附睾精子畸形率增加即有显著意义(P <0 0 5 ) ,12、2 4mg/kg体重剂量组染毒后第 5周末附睾精子畸形率极明显升高 (P <0 0 1)。精子畸形种类主要以头部畸形为主 ,主要包括无钩、不定型、香蕉型。 [结论 ]丙烯腈能够引起早期精细胞遗传损伤 ,精子畸形数增加 ,从而影响雄性小鼠的生殖功能。
To investigate the toxic effect of acrylonitrile on germ cells of male mice. [Methods] Male ICR mice were injected intraperitoneally with 0, 25, 5 and 10 mg / kg body weight for 5 days. After 2 weeks, the animals were sacrificed to observe the early spermatid in testis. , 12,2 4mg / kg body weight after continuous gavage 5d after the end of each weal some animals were killed for 5 weeks, analysis of epididymal sperm deformity rate. [Results] The results of early sperm micronucleus test showed that there was a dose-response relationship between the micronuclei rate and the dose of irradiation (y = 0 2 70x-0 816, r = 0 988). The results of sperm deformity test showed that the sperm deformity rate of 2 4mg / kg group increased significantly from week 2 to week 2 (P <0.05), and the sperm deformity of epididymal sperm of 12 and 24 mg / kg body weight group The rate was significantly higher (P <0.01). The main types of sperm deformity head deformity, mainly including no hook, not stereotypes, banana type. [Conclusion] Acrylonitrile can cause early spermatogonial damage and increase the number of sperm deformity, thus affecting the reproductive function of male mice.