论文部分内容阅读
目的探讨PUMA蛋白在依托泊苷诱导的宫颈癌细胞凋亡中的作用。方法采用MTT方法检测依托泊苷对宫颈癌细胞系Si Ha细胞活力的影响。通过Annexin-V/PI双染检测和光学显微镜形态观察检测依托泊苷对宫颈癌Si Ha细胞凋亡的影响。通过转染si RNA干扰PUMA的表达,检测低表达PUMA后,宫颈癌细胞对依托泊苷的敏感性。通过免疫印迹方法检测PUMA干扰后PUMA的表达水平。结果依托泊苷显著抑制宫颈癌细胞的活力和诱导其凋亡,且随着浓度和时间的增加而细胞活力依次减少而凋亡率依次升高,且光学显微镜下有明显的细胞皱缩等凋亡形态。当转染PUMA的si RNA后,依托泊苷诱导的细胞凋亡显著减少。结论 PUMA在依托泊苷诱导宫颈癌细胞凋亡中起了重要作用,它能够增强宫颈癌细胞对依托泊苷的敏感性。
Objective To investigate the role of PUMA in the apoptosis of cervical cancer cells induced by etoposide. Methods The effect of etoposide on the cell viability of cervical cancer cell line Si Ha was examined by MTT assay. The effect of etoposide on the apoptosis of Si Ha cells was detected by Annexin-V / PI double staining and optical microscopy. Transfection of si RNA interfered with the expression of PUMA to detect the sensitivity of cervical cancer cells to etoposide after low expression of PUMA. The expression of PUMA after PUMA interference was detected by Western blotting. Results Etoposide significantly inhibited the viability and induced apoptosis of cervical cancer cells. With the increase of concentration and time, the cell viability decreased and the apoptotic rate increased in turn, and obvious cell shrinkage was observed under light microscope Dead shape. When transfected with PUMA si RNA, etoposide-induced apoptosis was significantly reduced. Conclusion PUMA plays an important role in the apoptosis of cervical cancer cells induced by etoposide, which can enhance the sensitivity of cervical cancer cells to etoposide.