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目的研究依达拉奉对脂多糖(LPS)诱导的大鼠皮质神经元凋亡的保护作用。方法 90只健康成年雄性SD大鼠随机分为空白对照组、生理盐水组和依达拉奉组,每组按造模后的时间不同再分成5个亚组,比较各组大鼠的神经病学评分、诱导型一氧化氮合酶(iNOS)和肿瘤坏死因子-α(TNF-α)表达。48 h时,用TUNEL标记法检测神经细胞凋亡情况。结果与空白对照组相比,生理盐水组和依达拉奉组的神经病学评分明显降低(P均<0.05)。生理盐水组和依达拉奉组的iNOS、TNF-α表达明显增高(P均<0.05)。24 h后,依达拉奉组iNOS和TNF-α表达均明显小于生理盐水组,差异有显著统计学意义(P均<0.05)。此外,依达拉奉组的细胞凋亡少于生理盐水组。结论 LPS侧脑室注射能促使皮质神经元凋亡,可能与氧化应激有关,依达拉奉能有效减轻此损伤。
Objective To study the protective effect of edaravone on lipopolysaccharide (LPS) -induced apoptosis of cortical neurons in rats. Methods 90 healthy adult male Sprague-Dawley rats were randomly divided into blank control group, normal saline group and edaravone group. Each group was divided into 5 subgroups according to different time after modeling. Neurology Score, inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α) expression. At 48 h, neuronal apoptosis was detected by TUNEL labeling. Results Compared with the blank control group, neurological scores of the saline group and edaravone group were significantly lower (all P <0.05). The expressions of iNOS and TNF-α in saline group and edaravone group were significantly increased (all P <0.05). After 24 h, the expression of iNOS and TNF-α in edaravone group was significantly lower than that in saline group, the difference was statistically significant (all P <0.05). In addition, edaravone group less apoptosis than saline group. Conclusion Intraventricular injection of LPS can induce apoptosis of cortical neurons, which may be related to oxidative stress. Edaravone can effectively reduce this injury.