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目的研究抗生素的免疫调节作用及其对肺纤维化的影响。方法在体外实验中利用流式细胞术检测了抗生素对树突状细胞(DCs)和B淋巴细胞成熟及抗生素刺激后的DCs与小鼠T淋巴细胞混合培养对T细胞极化的影响。在体内实验中,采用博莱霉素致肺纤维化小鼠模型,检测制霉菌素对肺纤维化的调节作用。结果制霉菌素等多种抗生素均能不同程度促进DCs和B细胞成熟,并通过对DCs的作用,促进T细胞向Th2型免疫极化。而且,制霉菌素加剧博莱霉素所致小鼠肺纤维化。结论制霉菌素等多种抗生素诱导抑制性免疫反应,其中制霉菌素加剧博莱霉素所致小鼠肺纤维化,促进博莱霉素所致肺部抑制性免疫微环境形成。
Objective To study the immunomodulatory effect of antibiotics and its effect on pulmonary fibrosis. Methods The effects of antibiotics on the polarization of T cells induced by the combination of DCs and B lymphocytes stimulated by antibiotics with DCs stimulated by antibiotics and mouse T lymphocytes were detected by flow cytometry in vitro. In vivo experiments, bleomycin-induced pulmonary fibrosis in mice model to detect nystatin on the regulation of pulmonary fibrosis. Results Nystatin and other antibiotics can promote the maturation of DCs and B cells to varying degrees and through the action of DCs, promote the T cell to Th2 immune polarization. Moreover, nystatin aggravates bleomycin-induced lung fibrosis in mice. Conclusions Nystatin and other antibiotics induce an inhibitory immune response. Nystatin aggravates bleomycin-induced pulmonary fibrosis in mice and promotes the formation of pulmonary inhibitory immune microenvironment induced by bleomycin.