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目的 探讨血管紧张素Ⅰ转换酶（ＡＣＥ） 基因插入／ 缺失（Ｉ／Ｄ） 多态性与非胰岛素依赖型糖尿病（ＮＩＤＤＭ） 及并发眼底病变的关系。方法 以ＡＣＥ基因１６ 内含子的１ 个２８７ｂｐ 的Ａｌｕ 序列为多态性标志，用ＰＣＲ方法扩增基因片段，６ ％ 非变性聚丙烯酰胺凝胶电泳检测ＰＣＲ 产物。结果１４９ 例ＮＩＤＤＭ 与１００ 例正常人基因型频率与等位基因频率差异无显著性。ＤＤ 型与Ｄ 等位基因在ＤＲ（ ＋ ） 组出现频率高，ＤＲ（ ＋ ） 组与ＤＲ（ － ） 组相比，基因型及等位基因分布差异具显著性。结论 ＡＣＥ 基因多态性与ＮＩＤＤＭ 并发眼底病变有关
Objective To investigate the relationship between angiotensin Ⅰ converting enzyme (ACE) gene insertion / deletion (I / D) polymorphism and non-insulin dependent diabetes mellitus (NIDDM) and complicated ocular fundus lesions. Methods A 287bp Alu sequence of ACE gene 16 intron was used as a marker of polymorphism. PCR was used to amplify the gene fragment. PCR products were detected by 6% non-denaturing polyacrylamide gel electrophoresis. Results There was no significant difference in genotype frequency and allele frequency between 149 cases of NIDDM and 100 cases of normal controls. The frequencies of DD and D alleles in DR (+) group were higher than those in DR (+) group and DR (+) group. There were significant differences in genotype and allele distribution between DR (+) group and DR (-) group. Conclusion ACE gene polymorphism is associated with NIDDM complicated with ocular fundus lesions