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采用MNNC诱变与自交(S_1～S_3)纯合的方法,从辣椒疫霉的游动孢子群体中,筛选出1株带有抗霜脲氰标记的产毒缺陷突变体、2株抗甲霜灵的产毒突变体。产毒缺陷菌株和产毒菌株均对茄门甜椒致病,但产毒缺陷菌株的致病力降低一半,证明辣椒疫霉的致病显症过程与毒素作用有关,病菌毒素是重要的致病因子。此外,突变菌株的抗药性和产毒缺陷表型在无性游动孢子后代和有性卵孢子后代群体中均可稳定遗传。在姐妹配对F_2代卵孢子群体中,辣椒疫霉毒素产生和对甲霜灵的抗性为不完全显性基因所控制,而对霜脲氰的抗性为完全显性基因所控制。
A method of homozygous MNNC mutagenesis and selfing (S_1 ~ S_3) was used to screen a strain of toxigenic deformity-producing mutants with cytochromes and two anti-A Cream spirit of toxigenic mutants. Both the toxin producing strain and the toxigenic strain are pathogenic to the eggplant bell pepper, but the pathogenicity of the toxigenic strain is reduced by half, demonstrating that the pathogenic manifestation of Phytophthora capsici is related to the toxin action, and the pathogenic toxin is important Etiological factor. In addition, the resistant and detoxification phenotypes of the mutant strains were stable and inheritable in the cloned offspring of asexual zygotic and sexual ovary progeny. In sister-paired F_2 generation oospores population, the production of capsid and the resistance to metalaxyl were controlled by imperfect dominant genes, while the resistance to cymoxanil was controlled by the dominant gene.