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[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment,and provide reference for further development and use of Myrica ruba leaves. [Methods]Pigment of Myrica ruba leaves was extracted by ethanol extraction method. Absorbance was measured at wavelength of 300 nm. Effects of ethanol volume fraction,solid-to-liquid ratio,temperature,and extraction time were studied. L_9( 3~3) orthogonal experiment was carried out to optimize the extraction process,and DPPH scavenging ability was measured.[Results]The optimum extraction process of Myrica ruba leaf pigment was as follows: the ethanol volume fraction was 50%; the solid-to-liquid ratio was 1: 60( w/v),extraction temperature 70℃; extraction time was 60 min; after the concentration of purified Myrica ruba leaf pigment reached 0. 4455 mg/mL,the DPPH scavenging ability was basically the same as the same concentration Vc.[Conclusions] The experiment indicates that the optimized extraction process has a high yield rate,and Myrica ruba leaf pigment has high anti-oxidant activity.
[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment, and provide reference for further development and use of Myrica ruba leaves. [Methods] Pigment of Myrica ruba leaves was extracted by ethanol extraction method. Absorbance was measured at wavelength of 300 nm. Effects of ethanol volume fraction, solid-to-liquid ratio, temperature, and extraction time were studied. L_9 (3 ~ 3) orthogonal experiment was carried out to optimize the extraction process, and DPPH scavenging ability was measured. The solid-to-liquid ratio was 1: 60 (w / v), extraction temperature 70 ° C; extraction time was 60 min ; after the concentration of purified Myrica ruba leaf pigment reached 0. 4455 mg / mL, the DPPH scavenging ability was basically the same as the same concentration Vc. [Conclusions] The experiment indicates that the optimized extraction process h as a high yield rate, and Myrica ruba leaf pigment has high anti-oxidant activity.