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目的 探讨三七皂甙单体Rg1 、Rb1 (三七Rg1 、Rb1 )在脑损伤时对脑细胞凋亡和分泌型磷脂酶A2 (secretoryphospholipaseA2 ,sPLA2 )及其相关介质的影响。方法 用线栓法制成大脑中动脉缺血再灌注 (middlecerebralarteryischemia reperfusion ,MCA IR)模型 ,用原位末端标记 (TUNEL)法检测脑细胞凋亡 ,用免疫组织化学法检测sPLA2 在脑细胞中的表达 ,用3H标记大肠杆菌膜为底物的液闪方法和酶联免疫吸附测定 (ELISA)法 ,分别检测血清中sPLA2 活性和肿瘤坏死因子 α(TNF α)、前列腺素E2 (PGE2 )水平。结果 在大鼠MCA IR脑损伤时 ,三七Rg1 、Rb1 明显降低凋亡细胞数和血清中sPLA2 、TNF α、PGE2 水平及sPLA2 在脑细胞中的表达 ,与对照组相比 ,差异有显著性意义 (P <0 .0 1)。结论 三七Rg1 、Rb1 对MCA IR脑损伤有良好的保护作用 ,其机制在于抑制sPLA2 的激活、表达及其相关介质的水平。
Objective To investigate the effects of notoginsenoside Rg1, Rb1 (Panax Rg1, Rb1) on brain apoptosis and secretory phospholipase A2 (sPLA2) and its related media in brain injury. Methods Middle cerebral artery ischemia reperfusion (MCA IR) model was established by suture method. The apoptosis of brain cells was detected by TUNEL method. The expression of sPLA2 in brain cells was detected by immunohistochemistry. The activity of sPLA2 and the levels of tumor necrosis factor-alpha (TNF-alpha) and prostaglandin E2 (PGE2) in serum were measured by liquid scintillation and enzyme-linked immunosorbent assay (ELISA) using 3H-labelled E. coli membrane as a substrate. Results In rat MCA IR brain injury, Panax pseudoginseng Rg1 and Rb1 significantly decreased the number of apoptotic cells and serum levels of sPLA2, TNFα, PGE2 and sPLA2 in brain cells. There was a significant difference compared with the control group. Significance (P < 0.01). Conclusion Sanqi Rg1 and Rb1 have a good protective effect on brain damage induced by MCA. Its mechanism is to inhibit the activation and expression of sPLA2 and its related mediators.