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利用新一代高通量测序技术,对猕猴桃雌花和雄花中表达的小RNA进行了测序,分别得到雌花18 408 610条序列和雄花11 191 469条序列。通过生物信息学分析,共鉴定和预测得到39个保守mi RNA家族和400个新的mi RNA家族,其中有170个mi RNA家族在雌、雄花样品间显著差异表达。对差异表达mi RNA进行靶基因的预测及注释,结果显示,靶基因产物具有包含核苷三磷酸水解酶的磷酸环结构域的mi RNA数量最多。在猕猴桃25号连锁群(Chr25)上共预测得到3个mi RNA,其中novel-ach-mi R362的靶基因Achn298021可能与猕猴桃花的性别发育有关。
By using a new generation of high-throughput sequencing technology, the small RNAs expressed in female and male flowers of Actinidia chinensis were sequenced, and 18,408 610 and 11 191 469 sequences of male flowers were obtained respectively. By means of bioinformatics analysis, 39 conserved mi RNA families and 400 new mi RNA families were identified and predicted. Among them, 170 mi RNA families were significantly differentially expressed between female and male flower samples. The predicted and annotated target genes of differentially expressed miRNAs showed that the target gene products had the highest number of miRNAs with the phosphate ring domain containing nucleoside triphosphatase. A total of three mi RNAs were predicted on the Chromosome 25 (Chr25), and the target gene Achn298021 of novel-ach-mi R362 may be related to the sex development of Actinidia.