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目的探讨中药神经生长液对百草枯(PQ)诱导的帕金森病(PD)模型鼠的治疗作用。方法成年雄性SD大鼠40只,随机分为阴性对照组(n=10)、PQ模型组(n=10)、多巴丝肼片(美多芭)治疗组(n=10)、中药治疗组(n=10)。腹腔注射PQ制备PD模型,通过灌胃的方法对模型进行中药和美多芭治疗,以生理盐水为对照。治疗前后通过转棒实验、旷场实验和滚筒实验检测大鼠行为学变化,并记录大鼠的体重;采用酪氨酸羟化酶(TH)免疫染色检测中脑黑质多巴胺能神经元,计数并进行统计学分析。结果 1.模型制作后,与阴性对照组相比,模型动物体重显著降低(P<0.001)。然而,随着时间的延长,大鼠的体重不断增加,各组之间差异无显著性(P>0.05)。2.行为学检测结果显示,模型动物的转棒时间和滚筒时间较阴性对照组显著减少,旷场实验中的运动路程减少、静止时间延长,差异具有统计学意义(P<0.001)。灌胃1周后中药组和美多芭组在转棒实验、旷场实验和滚筒实验较模型组均有好转,差异具有统计学意义(P<0.001或P<0.01)。灌胃2周后中药组和美多芭组在转棒实验和旷场实验的路程比模型组显著增加,旷场实验中的静止时间比模型组显著减少,差异具有统计学意义(P<0.001)。3.免疫组织化学结果显示,灌胃1周时中药组TH阳性细胞数与阴性对照组相比减少(P<0.05),但与模型组相比显著增加(P<0.001),与美多芭组相比明显增加(P<0.01);灌胃2周的中药组与模型组相比,TH阳性细胞数显著增加(P<0.001),与美多芭组相比有所增加(P<0.05)。4.免疫荧光结果显示,中药组的TH阳性细胞数和细胞形态比模型组和美多芭组要多且完整,纤维多且长,与阴性对照组差异无显著性。结论中药神经生长液对PQ诱导的PD模型鼠有一定的治疗作用。
Objective To investigate the therapeutic effect of traditional Chinese medicine nerve growth fluid on paraquat-induced Parkinson’s disease (PD) model rats. Methods Forty adult male Sprague Dawley rats were randomly divided into 4 groups: n = 10, PQ group (n = 10), domperidone group (n = 10) Group (n = 10). PD model was prepared by intraperitoneal injection of PQ. The model was treated with traditional Chinese medicine and metoprolol by gavage, with saline as control. Before and after treatment, the behavioral changes of rats were detected by rotating bar test, open-field test and roller test, and the body weight of rats was recorded. The substantia nigra dopaminergic neurons were detected by tyrosine hydroxylase (TH) And statistical analysis. Results 1. After model making, the body weight of the model animals was significantly lower than that of the negative control group (P <0.001). However, with the extension of time, the body weight of rats continued to increase, with no significant difference between the groups (P> 0.05). The results of behavioral testing showed that the model animals had significantly less rotarod and roller time than those of the negative control group, decreased the distance traveled in the open field test, prolonged rest time, the difference was statistically significant (P <0.001). After 1 week of gavage, the traditional Chinese medicine group and the metopil group improved compared with the model group in rotarod test, open field test and roller test, the difference was statistically significant (P <0.001 or P <0.01). After 2 weeks of gavage, the distance between experimental group and open-field test in Chinese medicine group and Medicago group significantly increased than that in model group, and the resting time in open field test significantly decreased compared with model group (P <0.001) . Immunohistochemistry results showed that the number of TH-positive cells decreased significantly (P <0.05) compared with the negative control group, but significantly increased compared with the model group (P <0.001) (P <0.01). Compared with the model group, the number of TH-positive cells increased significantly (P <0.001) ). Immunofluorescence results showed that TH-positive cells and cell morphology in TCM group were more and more complete than those in model group and metoprolol group, with more fibers and longer length, but no significant difference with negative control group. Conclusion Chinese medicine nerve growth fluid has a certain therapeutic effect on PQ-induced PD model rats.