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目的:探讨链霉亲和素修饰的CdSe–ZnS量子点对聚合酶链反应的影响。方法:将链酶亲和素修饰的量子点浓度进行稀释观察其对于聚合酶链反应体系的浓度效应;反应体系中含有一定浓度的链酶亲和素修饰的量子点,在不同的退火温度下进行扩增,观察链酶亲和素修饰的量子点对退火温度的影响;将不同浓度的BSA加入到含有一定浓度的链酶亲和素修饰的量子点的反应体系中,观察BSA的影响作用;将不同公司的Taq酶与不同浓度链酶亲和素修饰的量子点组合,观察对扩增体系的影响。结果:一定浓度链霉亲和素修饰的量子点可以促进聚合酶链反应的扩增效率,拓宽PCR的退火温度范围;BSA可以逆转链霉亲和素修饰的量子点对于PCR的优化作用;量子点可能是与Taq酶相互作用,来影响PCR的扩增效能。结论:一定浓度的链霉亲和素修饰的量子点可以优化PCR的反应体系。
Objective: To investigate the effect of streptavidin-modified CdSe-ZnS quantum dots on polymerase chain reaction. Methods: The concentration of streptavidin-modified quantum dots was diluted to observe its concentration effect on the polymerase chain reaction system. The reaction system contained a certain concentration of streptavidin-modified quantum dots at different annealing temperature The effect of streptavidin-modified quantum dots on the annealing temperature was observed. Different concentrations of BSA were added to the reaction system containing a certain concentration of streptavidin-modified quantum dots to observe the effect of BSA ; Taq enzyme from different companies with different concentrations of streptavidin modified quantum dots combination to observe the impact on the amplification system. Results: Quantum dots modified with streptavidin could promote the amplification efficiency of polymerase chain reaction (PCR) and broaden the annealing temperature range of PCR. BSA can reverse the optimization of PCR for streptavidin-modified quantum dots. Quantum The point may be the interaction with Taq enzyme, to affect the PCR amplification efficiency. Conclusion: A certain concentration of streptavidin modified quantum dots can optimize the PCR reaction system.