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236对SSR引物中共有74对在小麦(Tritiumaestivum)抗、感亲本之间有多态性,多态性频率达31.7%。对小麦望水白/安农8455群体的SSR分析表明,在2年资料中都出现的与抗小麦赤霉病(Fusariumheadblight)连锁的SSR标记有14个,主要分布在染色体3B和2A上。利用MapManagerQTX软件构建连锁图和QTL定位,有38个标记主要分布于5条染色体上(3B、2A、5A、6B和7B);根据3年的抗性资料分析,3B上的QTL分别位于XBarc133~Xgwm389、XBarc133~Xgwm389和Xg-wm533.1~Xgwm493之间,LOD为2.58、6.49和2.45,分别能解释10%、23%和9%的表型变异。2001年和2003年的抗性资料分析表明,2A上存在2个抗性QTL,都位于Xgwm95~Xgwm372之间,LOD分别为2.82和2.93,均能解释11%的表型变异。
Among 236 pairs of SSR primers, 74 pairs had polymorphism among resistant and susceptible parents in Tritium aestivum, with a frequency of 31.7%. SSR analysis of wheat Wangshuibai / Annong 8455 population showed that there were 14 SSR markers linked to Fusarium head blight in 2 years data, mainly distributed on chromosomes 3B and 2A. According to the 3-year resistance data analysis, the QTLs on 3B locates in XBarc133 ~ XBarc133 and QTLs on XBarc133 ~ Xgwm389, XBarc133 ~ Xgwm389 and Xg-wm533.1 ~ Xgwm493 with LODs of 2.58, 6.49 and 2.45, respectively, which could explain phenotypic variation of 10%, 23% and 9% respectively. The analysis of resistance data in 2001 and 2003 showed that there were 2 resistant QTLs on 2A, all located between Xgwm95 ~ Xgwm372, with LODs of 2.82 and 2.93, respectively, which could account for 11% of phenotypic variation.