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研究优良品种木薯“ND-50”无性系组织培养技术。以木薯品种“ND-50”带腋芽的茎段为外植体进行组织培养,在芽的增殖和继代生长两个阶段进行观察研究。结果表明,2/3MS+6-BA 1.0 mg/L+IAA1.0 mg/L培养基较适合作为组培苗的增殖培养基;2/3MS+KT 1.0 mg/L+NAA0.05 mg/L作为组培苗的壮苗培养基较好。通过“ND-50”组织培养研究,为木薯的快速繁育和推广提供参考依据。
Study on Cultivation Techniques of Good Variety Cassava “ND-50” Clonal. The cauliflower cultivar “ND-50” with axillary buds was used as explants for tissue culture. The proliferation and secondary growth of buds were observed in two stages. The results showed that 2 / 3MS + 6-BA 1.0 mg / L + IAA1.0 mg / L medium was more suitable for proliferation of tissue culture medium; 2/3 MS + KT 1.0 mg / L + NAA0.05 mg / Strong tissue culture seedlings as a tissue culture medium is better. Through “ND-50 ” tissue culture research, provide a reference for the rapid breeding and promotion of cassava.