论文部分内容阅读
为观察庆大霉素损伤后豚鼠壶腹感觉细胞损伤与修复对前庭功能的影响,探讨前庭感觉上皮细胞损伤后自发修复与前庭功能恢复的关系。实验组动物连续12天皮下注射125mg/kg 庆大霉素,应用正旋摆动实验和旋转实验连续观察前庭功能的变化,结合不同存活时间动物水平半规管壶腹嵴的扫描电镜观察和半薄切片壶腹嵴顶部不同类型毛细胞的定量分析。结果示:停药第22天的10只动物中有4只动物前庭功能有一定程度的恢复.但仍有明显前庭功能低下,其中两只存活至100天。前庭功能无进一步恢复。停药后第1、3天,Ⅰ型毛细胞明显减少。停药后第22天、100天.Ⅰ型毛细胞数目仍然很少,Ⅱ型毛细胞明显增多(其中包括在形态上类似Ⅱ型毛细胞的新生的感觉细胞)。将正常和停药后不同时间豚鼠两种类型毛细胞数量变化与相对应半规管功能进行相关分析,水平半规管壶腹嵴顶部Ⅰ型毛细胞数与豚鼠半规管功能呈显著正相关,而Ⅱ型毛细胞数与半规管功能无显著相关性。本研究不能说明庆大霉素损伤后再生修复的前庭感觉细胞具有恢复正常生理功能的作用。
To observe the effects of gentamycin on the vestibular function after guinea pig ampulla sensory cell injury and repair, and to explore the relationship between spontaneous repair and vestibular function recovery after vestibular sensory epithelial cell injury. Animals in experimental group were injected subcutaneously 125 mg / kg gentamicin continuously for 12 days, and the changes of vestibular function were observed continuously by using positive-swinging and rotating experiments. Scanning electron microscope (SEM) and semi-thin slicing pot Quantitative analysis of different types of hair cells at the top of the ventral. The results showed that: 4 of 10 animals on the 22nd day of withdrawal had a certain degree of restoration of vestibular function, but there was still significant loss of vestibular function, of which two survived to 100 days. Vestibular function without further recovery. On day 1 and day 3, type Ⅰ hair cells decreased significantly. On day 22 and day 100 after discontinuation, the number of type I hair cells is still small, and type II hair cells are significantly increased (including newly born sensory cells that morphologically resemble type II hair cells). The changes of the number of hair cells in two types of guinea pigs at different time points after normal and stopping treatment were correlated with the function of semicircular canal. The number of type Ⅰ hair cells at the top of ampulla in horizontal semicircular canal had a significant positive correlation with the function of semicircular canal in guinea pigs, while the type Ⅱ hair cells No significant correlation between semicircular canal function and number. This study can not explain the role of vestibular sensory cells regenerating and repairing after gentamicin has the function of restoring normal physiological functions.