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为了提高蛇葡萄素的溶解性和抗肿瘤活性,以普朗尼克F127和D-α-维生素E聚乙二醇1000琥珀酸酯为复合载体材料,采用薄膜水化法制备蛇葡萄素纳米胶束,考察蛇葡萄素纳米胶束的最佳工艺条件和理化参数。采用MTT法,比较原料及纳米胶束制剂对MCF-7细胞增殖的抑制作用。结果表明,蛇葡萄素纳米胶束平均粒径为(22.6±0.5)nm,包封率为(80.42±1.13)%,载药量为(4.41±0.26)%。所制得的蛇葡萄素混合纳米胶束比蛇葡萄素原料的溶解度增加16倍,且在不同释放介质中8 h可累积释放药物90%以上,并能够显著抑制MCF-7细胞的增殖(P<0.01)。该混合纳米胶束可作为蛇葡萄素新的药物传递系统。
In order to improve the solubility and anti-tumor activity of snake grape, Snake grape nanometer micelles were prepared by the membrane hydration method using pluronic F127 and D-α-Vitamin E polyethylene glycol 1000 succinate as the composite carrier material , To investigate the optimum conditions and physical and chemical parameters of snake grape nanometer micelles. MTT assay was used to compare the inhibition of MCF-7 cell proliferation with the raw materials and nano-micelles. The results showed that the average particle size of snake grape nanocomposite was (22.6 ± 0.5) nm, the encapsulation efficiency was (80.42 ± 1.13)% and the drug loading was (4.41 ± 0.26)%. The solubility of the mixture of ampelopsin and nanomaterials increased by 16-fold compared with that of the same, and over 90% of the drug release could be released in different mediums for 8 h and significantly inhibited the proliferation of MCF-7 cells (P <0.01). The mixed nano-micelles can be used as a new drug delivery system of snake grape.